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Protozoa such as amoeba depend on diffusion as a means of excretion. They have a large surface area to volume ratio for efficient diffusion.

Nitrogenous waste and carbon (IV) oxide are highly concentrated in the organism hence they diffuse out. In amoeba excess water and chemicals accumulation in the contractile vacuole.

When it reaches maximum size the contractile vacuole moves to the cell membrane, bursts open releasing its contents to the surroundings.

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